simplechip human α satellite repeat primers (Cell Signaling Technology Inc)
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simplechip human α satellite repeat primers
Simplechip Human α Satellite Repeat Primers, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simplechip+human+%CE%B1+satellite+repeat+primers/SimpleChIP+Human+alpha+Satellite+Repeat+Primers/pmc12999499-256-23-30
Average 93 stars, based on 25 article reviews
Simplechip Human α Satellite Repeat Primers, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simplechip+human+%CE%B1+satellite+repeat+primers/SimpleChIP+Human+alpha+Satellite+Repeat+Primers/pmc12999499-256-23-30
Average 93 stars, based on 25 article reviews
simplechip human α satellite repeat primers - by Bioz Stars,
2026-10
93/100 stars
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1) Product Images from "Reduced cyclin D3 expression in erythroid cells protects against malaria"
Article Title: Reduced cyclin D3 expression in erythroid cells protects against malaria
Journal: Nature
doi: 10.1038/s41586-026-10110-9
Figure Legend Snippet: a , Top, vector content and expression of luciferase reporter genes driven by the extreme allele combinations: on the one hand the WT rs9349205-G|rs112233623-C (G|C) alleles on the other hand the derived rs9349205-A| rs112233623-T (A|T) alleles of the CCND3 enhancer in the erythroid cell line HUDEP-2, with empty vector as a control construct. Below, histograms show averages of the relative luminescence activity of the combinations of the two extreme alleles at rs9349205 and rs112233623 described above in absence (-) or presence of GATA1 and FOG-expressing plasmids; SMAD3 and p300 expressing plasmids; SMAD3 expressing plasmid alone; or empty expression vector. All values are plotted relative to the WT (G|C) construct. Note that the activity of the empty pGL4 vector is barely detectable. The mean ± s.e.m is shown (n = 7 (vectors); n = 3 (vectors + SMAD3), n = 3 (vectors+SMAD3 + p300), n = 4 (vectors + GATA1 + FOG) biologically independent experiments). A two-sided Student’s t -test was used, with level of significance indicated by asterisks (*P < .05;**P < .01; ****P < .0001, ns, not significant). The statistical results for all comparisons are provided in Supplementary Table . b , In silico prediction of binding to rs112233623 allele variants, showing derived allele T hindering binding of SMAD3 while favouring GATA1 binding. c , d , Representative electrophoretic mobility shift assays (EMSA) showing binding of SMAD3 and GATA1 proteins with labelled oligonucleotide probes (*) containing the WT (C) or derived (T) allele of rs112233623 (n = 3 biologically independent experiments). Competitor unlabelled oligonucleotides were used at the indicated fold excess to demonstrate specificity of binding. c , SMAD3 binds to the WT rs112233623-C allele (lanes 2 and 8); is weakly supershifted by anti-SMAD3 antibody (lanes 3 and 9); and is competed away by an excess of unlabelled DNA oligonucleotides containing the WT rs112233623-C allele (lanes 10-12, at 25X, 50X and 100X respectively), but not by an excess of oligonucleotide containing the derived rs112233623-T allele (lanes 13-15, at 25X, 50X and 100X respectively). d , GATA1 binds to the rs112233623-T derived allele (lanes 2, 8 and 9), is supershifted by anti-GATA1 antibody (lane 3); and is competed away by an excess of unlabelled DNA oligonucleotides containing the derived rs112233623-T allele (lanes 10-12, at 25X, 50X and 100X respectively) compared to unlabelled DNA oligonucleotides containing the WT rs112233623-C allele (lanes 13-15, at 25X, 50X and 100X respectively). GATA1 does not appear to bind to the WT rs112233623-C allele (lane 5). The samples derive from the same experiment and, the gels were processed in parallel. For gel source data, see Supplementary Fig. . e , f , ChIP–qPCR for SMAD3 or GATA1 binding to the CCND3 enhancer region surrounding rs112233623 ( CCND3 ) and to a SimpleChIP Human α Satellite as negative control in erythroblasts derived from individuals homozygous for the rs112233623-T decrease of expression (DoE) allele versus the WT rs112233623-C allele (WT). e , ChIP was performed using an antibody against SMAD3, results are represented as percentage of input and nonspecific IgG used as negative control. The mean ± s.e.m is shown (n = 3 biologically independent experiments). A two-sided two-Sample t-test was used; significant differences are indicated (*P < .05; ns, not significant). The statistical results for the comparisons are provided in Supplementary Table . f , ChIP assays were conducted with an antibody against GATA1, results are represented as percentage of input and nonspecific IgG used as negative control (n = 2 biologically independent experiments).
Techniques Used: Plasmid Preparation, Expressing, Luciferase, Derivative Assay, Control, Construct, Activity Assay, In Silico, Binding Assay, Electrophoretic Mobility Shift Assay, ChIP-qPCR, Negative Control
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Negative Control:Article Title: STAT3-induced NCK1 elevation promotes migration of triple-negative breast cancer cells via regulating ERK1/2 signaling. Article Snippet: Background Noncatalytic region of tyrosine kinase 1 (NCK1) plays a key role in extracellular matrix degradation, which is required for the metastasis of triple-negative breast cancer (TNBC).. However, the role NCK1 plays in the metastatic progression of TNBC is unknown.. Methods and results Based on online databases, NCK1 was found to be highly expressed in TNBC as compared to normal breast-like subjects, which was also confirmed using TNBC cells and a tissue microarray. Article Title: Nuclear pyruvate kinase M2 complex serves as a transcriptional coactivator of arylhydrocarbon receptor Article Snippet: .. As a negative control of genome region, Binding Assay:Article Title: STAT3-induced NCK1 elevation promotes migration of triple-negative breast cancer cells via regulating ERK1/2 signaling. Article Snippet: Background Noncatalytic region of tyrosine kinase 1 (NCK1) plays a key role in extracellular matrix degradation, which is required for the metastasis of triple-negative breast cancer (TNBC).. However, the role NCK1 plays in the metastatic progression of TNBC is unknown.. Methods and results Based on online databases, NCK1 was found to be highly expressed in TNBC as compared to normal breast-like subjects, which was also confirmed using TNBC cells and a tissue microarray. Chromatin Immunoprecipitation:Article Title: Reduced cyclin D3 expression in erythroid cells protects against malaria Article Snippet: Immunoprecipitations were performed using 10 μg of an antibody against SMAD3 or GATA1, or 1 μg with nonspecific rabbit IgG as negative control (2729S Cell Signaling Technology), according to the manufacturer’s instructions, at 4 °C for 12–16 h. The next day, the immunocomplexes were rotationally incubated with 30 μl of ChIP-Grade Protein G Magnetic Beads for two hours at 4 °C and then washed three times using low-salt wash buffer and once with high-salt wash buffer at 4 °C for 5 min per wash. Chromatin was eluted by ChIP elution buffer for 30 min at 65 °C with gentle vortex mixing (1,200 rpm) and cross-links were reversed by treatment with 5 M NaCl and proteinase K for two hours at 65 °C. .. ChIP DNA was purified and subsequently quantified by qPCR using primers designed to amplify a region surrounding rs112233623 in the CCND3 enhancer and Article Title: Reduced cyclin D3 expression in erythroid cells protects against malaria. Article Snippet: After 48 h, luciferase activity was measured using a Synergy 2 plate reader (Biotek) with a Dual-Glo luciferase assay system (Promega) according to the manufacturer’s instructions. .. ChIP DNA was purified and subsequently quantified by qPCR using primers designed to amplify a region surrounding rs112233623 in the CCND3 enhancer and Purification:Article Title: Reduced cyclin D3 expression in erythroid cells protects against malaria Article Snippet: Immunoprecipitations were performed using 10 μg of an antibody against SMAD3 or GATA1, or 1 μg with nonspecific rabbit IgG as negative control (2729S Cell Signaling Technology), according to the manufacturer’s instructions, at 4 °C for 12–16 h. The next day, the immunocomplexes were rotationally incubated with 30 μl of ChIP-Grade Protein G Magnetic Beads for two hours at 4 °C and then washed three times using low-salt wash buffer and once with high-salt wash buffer at 4 °C for 5 min per wash. Chromatin was eluted by ChIP elution buffer for 30 min at 65 °C with gentle vortex mixing (1,200 rpm) and cross-links were reversed by treatment with 5 M NaCl and proteinase K for two hours at 65 °C. .. ChIP DNA was purified and subsequently quantified by qPCR using primers designed to amplify a region surrounding rs112233623 in the CCND3 enhancer and Article Title: Reduced cyclin D3 expression in erythroid cells protects against malaria. Article Snippet: After 48 h, luciferase activity was measured using a Synergy 2 plate reader (Biotek) with a Dual-Glo luciferase assay system (Promega) according to the manufacturer’s instructions. .. ChIP DNA was purified and subsequently quantified by qPCR using primers designed to amplify a region surrounding rs112233623 in the CCND3 enhancer and Real-time Polymerase Chain Reaction:Article Title: Reduced cyclin D3 expression in erythroid cells protects against malaria Article Snippet: Immunoprecipitations were performed using 10 μg of an antibody against SMAD3 or GATA1, or 1 μg with nonspecific rabbit IgG as negative control (2729S Cell Signaling Technology), according to the manufacturer’s instructions, at 4 °C for 12–16 h. The next day, the immunocomplexes were rotationally incubated with 30 μl of ChIP-Grade Protein G Magnetic Beads for two hours at 4 °C and then washed three times using low-salt wash buffer and once with high-salt wash buffer at 4 °C for 5 min per wash. Chromatin was eluted by ChIP elution buffer for 30 min at 65 °C with gentle vortex mixing (1,200 rpm) and cross-links were reversed by treatment with 5 M NaCl and proteinase K for two hours at 65 °C. .. ChIP DNA was purified and subsequently quantified by qPCR using primers designed to amplify a region surrounding rs112233623 in the CCND3 enhancer and Article Title: Reduced cyclin D3 expression in erythroid cells protects against malaria. Article Snippet: After 48 h, luciferase activity was measured using a Synergy 2 plate reader (Biotek) with a Dual-Glo luciferase assay system (Promega) according to the manufacturer’s instructions. .. ChIP DNA was purified and subsequently quantified by qPCR using primers designed to amplify a region surrounding rs112233623 in the CCND3 enhancer and Positive Control:Article Title: Investigating the Effects of a Small-Molecule Allosteric Inhibitor of Core-Binding Factor Subunit Beta in Human Osteosarcoma Article Snippet: Lyophilized ChIP primers were purchased through IDT (Integrated DNA Technologies, Coralville, IA), and reconstituted to 100 μM in nuclease-free water. .. |